Fig. 5. Correlation between intracellular L-glutamine levels and contents of amino acids produced from glutamine metabolism in C₂C₁₂ myotubes cultivated in the absence of glutamine (no addition and no addition plus glutamine synthase inhibitor) for 48 hours. Fig.s refer to the following amino acids: L-glutamine, L-alanine; L-aspartate, L-asparagine, and L-proline. The experimental groups are: (0 mM) no glutamine addition in the medium; (0 mM with glutamine synthase inhibitor) no glutamine addition in the medium with the glutamine synthase inhibitor (L-methionine sulfoximine - MSO at 0.1 mM). Results were analyzed using Pearson correlation (Pearson r>0.07).